Xu Wang's Research Skills
- Lab skills
Sample collection, DNA and RNA extraction, agarose gel, PCR primer design, PCR, RT-PCR, qPCR, 5’ and 3’-RACE, restriction enzyme analysis, STRs scanning, Sanger sequencing, Bisulfite-sequencing, Pyrosequencing, Pyro-Mark, Illumina library preparation for gDNA-seq, RNA-seq, WGBS-seq and ChIP-seq, Agilent SNP-typing array design and hybridization, NimbleGen seq-cap array hybridization.
- Computational/Statistics Skills
- · Unix shell scripting, Linux/Unix system administration, cluster computing, C and Perl programming, MySQL database applications;
· Statistical software: R, JMP and SAS.
- Bioinformatic Skills
· Nextgen sequencing data analysis (Illumina, SOLiD, 454);
· Microarray primer design (for expression, SNP-typing and seq capture chip);
· Genetic linkage analysis and segregation analysis;
· Phylogenetic tree construction;
· Population genetic analysis and QTL mapping.